In order to investigate whether activities of liver and serum adenosine triphosphatases(ATPases), ribonucleases (RNase) and 5´-nucleotidase were changed in liver disorders characterized by tissue damage, activities of hepatic and serum. ATPase, RNase and 5´-nucleotidase were determined in rats with hepatotoxicity induced by phenobarbital and CC14, and the results were compared with those of normal rats.
1) In rats with hepatotoxicity induced by phenobarbital and CCI4, activities of hepatic Mgt+-ATPase, Na+, K+-ATPase and Mg2+-rNa+, K+-ATPase were all significantly decreased and- activities of serum Mg2+-ATPase and Mg++ Na-, K+-ATPase were significantly increased. These results suggest the excretion of the ATPase from the damaged liver cells into the blood.
2) While activities of hepatic acid RNase measured with RNA as the substrate were significantly increased in rats with hepatotoxicity induced by phenobarbital and CC14, activities of hepatic alkaline RNase determined with RNA or poly C as the substrate were significantly decreased. In rats with hepatotoxicity induced by phenobarbital and CC14, activities of both acid and alkaline RNase in serum were significantly decreased when RNA g or poly C was used as the substrate,.but increased when poly A was used as the substrate. These results. indicate that changes in activities of hepatic and serum RNase occured in CC14 hepatotoxicity characterized- by tissue damage. However, no correlation was. observed between changes in RNase activities of liver and serum.
3) The activity of 5´-nucleotidase in liver was not changed in rats with hepatotoxicity induced by phenobarbital and CC14. On the other hand, the activity of the serum enzyme was significantly increased in rats with hepatotoxicity induced by phenobarbital and CC14. These results suggest that 5´-nucleotidase was released from the damaged liver cells into the blood without showing any significant fall in the activity of the hepatic enzyme.
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